Spin-Labeled Membranes
نویسندگان
چکیده
منابع مشابه
Spin-Labeled Membranes
In the next few minutes I shall present a very brief discussion of what the spin-label technique is, and how it may be used to study biological membranes, x, 2 A "spin label" is a synthetic organic paramagnetic free radical. Most spin
متن کاملInteraction of Spin-Labeled Lipid Membranes with Transition Metal Ions
The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Wheth...
متن کاملESR studies of spin-labeled membranes aligned by isopotential spin-dry ultracentrifugation: lipid-protein interactions.
Electron spin resonance (ESR) studies have been performed on spin-labeled model membranes aligned using the isopotential spin-dry ultracentrifugation (ISDU) method of Clark and Rothschild. This method relies on sedimentation of the membrane fragments onto a gravitational isopotential surface with simultaneous evaporation of the solvent in a vacuum ultracentrifuge to promote alignment. The degre...
متن کاملSpin-labeled hemoglobin.
In the present paper we describe the results of a preliminary study of the paramagnetic resonance spectrum of spin-labeled horse hemoglobin in solution. Our results provide strong evidence that on oxygenation the A chains undergo a significant structural change near the "reactive" sulfhydryl group (f693), a change not yet resolved by X-ray diffraction.' We also find features in the paramagnetic...
متن کاملSpin-labeled biomolecules.
are remarkably stable and inert,1 and show sharp, well-resolved, and simple paramagnetic resonance spectra2 that are sensitive to molecular motion,3 and, to a lesser extent, sensitive to polarity of the molecular environment.4 As shown below, such nitroxide radicals containing an isocyanate group can be bound to proteins and synthetic polypeptides in a manner similar to that used previously to ...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1969
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.54.1.277